Carcinoma of the uterine cerivx is the most common malignant tumor in Korean women Decades of epidemiologic studies ot determine the etiology of cancer of the cervix have supported the concept of cervical cancer being a multifactorial factors
such
as
age at first intercourse, multiple sexual partner, socioeconomic status, smoking and sexually transmitted infectious agents.
More than 10 years age HPV was hypothesized as a possible etiologic agnet in development of squamous cell carcinoma. More than about 10 HPV have been found in CIN or invasive cervical cancer among 60 different HPV types. HPV type 6 and 11 are
frequently
noted in benign wart of CIN I or CIN II, but HPV type 16 and 18 are considered oncogenic. In recent report , a very sensitive polymerase chain reaction (PCR) procedures capable of amplifing specific DNA sequence was recently developed for
detection
of
HPV DNA.
Cellular oncogenes play a specific role in normal cellular development and differentiation. Activation of these oncogenes in assumed to be associated with neoplastic growth of the tumors. In recent report. C-myc and H-ras amplification and
structural
alteration have been demonstrated in cervical carcinoma.
In this study, we examined the presence of HPV genome by PCR and the state of oncoprotein of c-myc and H-ras by immunohistochemistry in fifty-three cases of cervical tumor samples. A possible relation between the prognosis of disease and presence
of HPV
type 16, 18 or c-myc and H-ras oncoprotein expression was analysed.
@ES The results were as follows:
@EN 1. The detection rate of HPV type 16 was 67.9%(36/53 cases), while HPV 18 was not detected.
2. The proportion of carcinoma with HPV type 16 positivity significantly increased with the clinical stage, whereas no correlation was found between HPV type 16 positivity and the other clinical and histological parameters.
3. The positive rate of c-myc oncoprotein was 64.2%(34/53 cases) and of H-ras oncoprotein was 9.4%(5/53 cases).
4. There was statistically significant correlation between H-ras oncoprotein positivity and increasing age, whereas the other clinical and histological parameters showed no significance in c-myc and H-ras oncoproteins.
5. There was not significant correlation between HPV type 16 positivity and c-myc and H-ras oncoprotein positivity.
These results suggest that HPV DNA and c-myc and H-ras oncogenes may play an essential role in the induction of cervical carcinoma independently.
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